STERLIX® Müller-Hinton Ready To Use Agar (MHA) Plate

Product Details
Müller-Hinton agar (MHA) is a common culture medium used in microbiology laboratories, particularly for antibiotic susceptibility testing of bacteria. It was developed by J. Hinton in collaboration with E. Müller in the 1940s.

Müller-Hinton agar is a non-selective and non-differential medium with a standardized composition. It contains a combination of protein digests, starch, and beef extract that provide essential nutrients for bacterial growth. The agar itself acts as a solidifying agent to support colony formation.

MHA is widely used for the Kirby-Bauer disk diffusion method, which is employed to determine the susceptibility or resistance of bacteria to various antibiotics. In this method, paper disks impregnated with specific antibiotics are placed onto the surface of the MHA plate, and the growth inhibition zones around the disks are measured after incubation. The results help guide clinicians in choosing appropriate antibiotics for the treatment of bacterial infections.

The standardized composition of Müller-Hinton agar ensures consistent growth and reproducible results. Its low calcium and magnesium content minimize the inhibitory effects of these ions on antibiotic activity, allowing for accurate interpretation of susceptibility testing results.

Overall, Müller-Hinton agar provides a reliable and standardized medium for antibiotic susceptibility testing and is widely used in clinical microbiology laboratories.